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胶原纤维的解聚是决定其高值化用途的关键步骤，常用的酸碱法因伴随有大量胶原的水解使纤维解聚的可控性不佳。利用羊皮酸浸后皮样为供试材料，通过机械破碎-超声协同的物理法对酸皮中胶原纤维的解聚作用进行了实验，并采用SEM、FTIR、XRD、UV-vis、XPS、DSC对胶原纤维的微观形貌、分子结构以及热稳定性进行分析。结果表明，酸皮经机械破碎后，胶原纤维大部分解聚成相互独立交织的胶原原纤维，将其在离子水环境下功率为480 W超声7 min，胶原微原纤维进一步解聚可完全分散于水相，胶原微纤维分布颗粒分布均匀，经冷冻干燥后形成的三维薄层结构，热稳定较好，胶原分子三螺旋结构保持完整，基本无水解变性，使胶原纤维中活性基团数量和反应活性显著增加，研究为胶原纤维高值化应用的前期预处理提供了实践依据。
Abstract：The de-bundling of collagen fibers is a key step in determining their high-value applications.The commonly used acid-base method is accompanied by a large amount of collagen hydrolysis,which makes the controllability of fiber de-bundling poor. Using the acid-impregnated sheepskin as the test material,the de-bundled effect of collagen fibers in acid-impregnated skin was experimentally studied by a physical method of mechanical crushing and ultrasonic synergy. The microscopic morphology,molecular structure and thermal stability of collagen fibers were analyzed by SEM,FTIR,XRD,UV-vis,XPS and DSC. The results showed that after mechanical crushing of the acid-impregnated skin,most of the collagen fibers were depolymerized into independent and interwoven collagen fibrils. When they were ultrasonicated in an ionic water environment at a power of 480 W for 7 min,the collagen microfibrils were further de-bundled and could be completely dispersed in the aqueous phase. The collagen microfibrils were evenly distributed,and the three-dimensional thin-layer structure formed after freeze-drying had good thermal stability. The triple helix structure of the collagen molecule was maintained intact,with almost no hydrolytic denaturation,which significantly increased the number of the active groups and activity in the collagen fibers. The research provided a practical basis for the pretreatment of collagen fibers in high-value applications.
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基本信息:
DOI：10.13353/j.issn.1004.9533.20240910
中图分类号:TK6
引用信息:
[1]马宏瑞,阮萱颖,朱超,等.机械破碎-超声对皮胶原纤维解聚的影响[J].化学工业与工程,2026,43(01):61-69.DOI:10.13353/j.issn.1004.9533.20240910.
基金信息:
国家自然科学基金项目(22076113)
2026-01-15
2026-01-15